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KMID : 0385520080210050412
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Analytical Science & Technology
2008 Volume.21 No. 5 p.412 ~ p.423
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Optimization of solid-phase extraction for the liquid chromatography-tandem mass spectrometry analysis of basic drugs in equine urine
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Shin Hyun-Du
Yang Ji-Suk
Jung Mi-Hye
Kim Hyung-Seung
Hu Man-Bae
Kim Sang-Jin
Han Sang-Beom
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Abstract
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A procedure based on solid-phase extraction (SPE) followed by liquid chromatography-tandem mass spectrometry has been developed for the simultaneous analysis of 55 basic drugs in equine urine. The test scope covers diversified classes of drugs including some β-blockers, β-agonists, antihypotensives, CNS stimulants, sedatives, tranquilizers, antidepressants, antihypertensives and so on. LC-MS/MS separation and quantification was carried out in positive electrospray ionization and multiple reaction monitoring (MRM) mode. Four different brands of mixed mode cation exchange SPE sorbents; UCT XTRACT® XRDAH, Supelco DSC-MCAX®, Varian Bond Elut Certify® and Waters Oasis® MCX were compared. The UCT XTRACT® XRDAHthe best results in the preconcentration of samples, yielding relative recoveries higher than 80% except for terbutaline (41.3%), salbutamol (71.5%), heptaminol (70.7%), phenylpropanolamine (66.3%). Detection limits of the target drugs provided by the proposed analytical procedure were between 0.2~8.3 ng/mL. sorbent provided
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KEYWORD
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Basic drugs, Equine urine, SPE, LC-MS/MS
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